There are two types of color developers: general color developers and special effect color developers. According to whether the chemical composition of the test object is damaged, the developer is divided into a destructive developer and a non-destructive developer.
In the experiment, we generally mix it into liquids A and B. When the color needs to be developed, add A and B separately. When the mixed solution reacts with the complex (antigen-antibody complex) in the experiment, it will produce a color development effect .
Solution A is generally composed of: PBS buffer, citric acid, ED-TA ethylene diamine tetraacetic acid, ProcLin-300, hydrogen peroxide (hydrogen peroxide is too volatile and easy to lose, we are now using urea peroxide to ｒｅｐｌａｃｅ it, The effect is pretty good) composition.
Solution B is generally composed of: PBS buffer, citric acid, ED-TA ethylenediaminetetraacetic acid, ProcLin-300, sodium thiosulfate, and the like.
Since the reaction of the color development solution continues to react once it starts, it is necessary to stop the reflection when the desired effect is reached, and a stop solution needs to be added.